RGS4 is arginylated and degraded by the N-end rule pathway in vitro

The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue. We used an expression-cloning screen to search for mouse proteins that are degraded by the ubiquitin/proteasome-dependent N-end rule pathway in a reticulocyte lysate. One substrate thus identified was RGS4, a member of the RGS family of GTPase-activating proteins that down-regulate specific G proteins. A determinant of the RGS4 degradation signal (degron) was located at the N terminus of RGS4, because converting cysteine 2 to either glycine, alanine, or valine completely stabilized RGS4. Radiochemical sequencing indicated that the N-terminal methionine of the lysate-produced RGS4 was replaced with arginine. Since N-terminal arginine is a destabilizing residue not encoded by RGS4 mRNA, we conclude that the degron of RGS4 is generated through the removal of N-terminal methionine and enzymatic arginylation of the resulting N-terminal cysteine. RGS16, another member of the RGS family, was also found to be an N-end rule substrate. RGS4 that was transiently expressed in mouse L cells was short-lived in these cells. However, the targeting of RGS4 for degradation in this in vivo setting involved primarily another degron, because N-terminal variants of RGS4 that were stable in reticulocyte lysate remained unstable in L cells

Weight Spectrum of Quasi-Perfect Binary Codes with Distance 4

We consider the weight spectrum of a class of quasi-perfect binary linear codes with code distance 4. For example, extended Hamming code and Panchenko code are the known members of this class. Also, it is known that in many cases Panchenko code has the minimal number of weight 4 codewords. We give exact recursive formulas for the weight spectrum of quasi-perfect codes and their dual codes. As an example of application of the weight spectrum we derive a lower estimate for the conditional probability of correction of erasure patterns of high weights (equal to or greater than code distance).Comment: 5 pages, 11 references, 2 tables; some explanations and detail are adde